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A Simple Method of Preparing “Coated” Red Blood Cells for Use as a Control in Antiglobulin Testing
Author(s) -
Fresco Raoul,
Busch Shirley,
Hanson Mary Lu,
Huestis Douglas W.
Publication year - 1964
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1964.tb02869.x
Subject(s) - antiserum , antibody , coombs test , incubation , red blood cell , immunology , red cell , chemistry , medicine , biochemistry
Red blood cells may be “coated” with antibody by means of incubation with an appropriately selected antiserum. The coated cells are then washed, resuspended in a much‐diluted group AB serum, and used to detect the free antiglobulin remaining in negative direct or indirect Coombs tests. A method of preparing such cell suspensions is given, and their use to detect false negative antiglobulin tests is discussed.