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The Measurement of Glycerol in the Presence of Citrate
Author(s) -
Greenwalt Tibor J.,
Steane Edwin A.
Publication year - 1964
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1964.tb02860.x
Subject(s) - glycerol , chemistry , chromatography , precipitation , alcohol , barium hydroxide , inorganic chemistry , biochemistry , physics , meteorology
A method for determining glycerol in citrated blood is described. It was found that citrate ions produce interfering turbidity when the procedure recommended by Bailey is applied to glycerol‐citrate‐blood mixtures. The addition of absolute alcohol during deproteinization results in the precipitation of citrates and eliminates the need for zinc sulfate and its subsequent removal by the addition of excess barium hydroxide. The average recovery of glycerol added to blood‐citrate mixtures is 99 per cent.