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Electronic Particle Counting Applied to the Quantitative Study of Red Cell Agglutination
Author(s) -
Bowdler A. J.,
Swisher Scott N.
Publication year - 1964
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1964.tb02852.x
Subject(s) - agglutination (biology) , coulter counter , red cell , cell counting , particle size , cell size , range (aeronautics) , population , chemistry , cell , medicine , materials science , immunology , biology , microbiology and biotechnology , antibody , biochemistry , environmental health , cell cycle , composite material
Quantitation of red cell agglutination can be made by counting the residual free cells remaining after agglutination and comparing the count with the pre‐agglutination red cell count. Most previous studies have used visual hemocytometry for this purpose. The use of the Coulter Model B electronic particle counter has been investigated and is shown to be a practical alternative. The problem of counting a free cell population overlapped in size by small aggregates is approached by two methods. In the first, a correction is made for aggregates present in the size range of single red cells based on the change in mean particle volume which results from the presence of cell clumps. In the second, red cells are counted in a size range in which aggregates are unlikely to occur. The methods are evaluated and their extension to provide further data on the pattern of red cell aggregation in agglutination is indicated.