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Behavior of Incomplete Antibodies in Quantitative Hemagglutination Reactions
Author(s) -
Solomon Joel M.
Publication year - 1964
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1964.tb02838.x
Subject(s) - centrifugation , agglutination (biology) , antibody , chemistry , hemagglutination , bovine serum albumin , saline , isoantibodies , steric effects , chromatography , immunology , biology , stereochemistry , endocrinology
The increase in agglutinating activity of group O sera following centrifugation has been related to incomplete, cross‐reactive antibodies in these sera. The effect is thought to be due to steric interference of smaller 7S, cross‐reactive molecules by the larger 19S agglutinins rather than to any lack of equilibrium in the agglutinating system. An increase in activity was also demonstrated with anti‐Rh sera containing saline agglutinins and incomplete antibodies following centrifugation. With anti‐Rh sera, centrifugation at 17,750 × g for 7.5 minutes was required to produce the same effect apparent with group O sera centrifuged at 100 × g for two minutes. High speed centrifugation produced saline agglutination with incomplete anti‐Rh antibodies equivalent to that produced in 20 per cent bovine albumin.