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The Reactions between Blood Group Antibodies and Antisera to Purified Immunoglobulins *
Author(s) -
Abelson Neva M.,
Rawson Arnold J.
Publication year - 1963
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1963.tb04675.x
Subject(s) - antibody , gamma globulin , antiserum , serology , globulin , beta globulins , chemistry , blood proteins , microbiology and biotechnology , immunology , biology , biochemistry
Rabbit antibody to chromatographically separated 7S gamma globulin proved to be a potent anti‐gamma globulin reagent, capable of potentiating the serologic activity of blood group antibodies of the 7S gamma globulin class without dependence on complement. Purified proteins of the beta 2A class caused the production of weak antibodies to gamma globulin and to complement as well as to beta 2A globulin. Specific anti‐beta 2A serum prepared by absorbing this reagent with cord blood serum appeared incapable of potentiating the serologic activity of the blood group antibodies tested. Purified proteins of the beta 2M class caused the production of antibodies to gamma globulin and to complement as well as to beta 2M globulin. Such antisera were of broad spectrum activity. Specific anti‐beta 2M serum prepared by absorption with cord blood serum was found capable of potentiating the serologic activity of only certain examples of anti‐Lewis agglutinins. It can be questioned whether this potentiation was due to specific anti‐19S activity, to cross‐reactivity with 7S gamma globulin or to other factors.