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Damage to Red Cells Due to Their Collection and Storage in ACD
Author(s) -
Bishop Charles
Publication year - 1963
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1963.tb04639.x
Subject(s) - glycolysis , in vitro , laboratory flask , red blood cell , chemistry , red cell , biochemistry , phosphate , anticoagulant , blood preservation , andrology , metabolism , medicine
Experiments were performed using aliquots of the same blood incubated at 37 C. in spinner flasks under 95%N 2 – 5% CO 2 . Various anticoagulant systems and p H's were compared, using the rate of glycolysis and ATP levels as criteria of metabolic activity. Citrate and lower p H's were detrimental to glycolysis and the maintenance of red cell ATP levels. Non‐citrate systems that were closer to normal blood p H were superior to ACD, by the criteria used in this in vitro study. The addition of phosphate, as in the citrate‐phosphate‐dextrose system, appeared beneficial.