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Biochemical and Electron Microscopic Study of Preserved Erythrocytes
Author(s) -
Rimon A.,
Rimon S.,
Da D.
Publication year - 1963
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1963.tb04613.x
Subject(s) - hemolysis , ultrastructure , lesion , membrane , red cell , population , electron micrographs , chemistry , cell , biophysics , electron microscope , biology , biochemistry , pathology , anatomy , medicine , immunology , physics , environmental health , optics
The age population distribution as judged by the ultrastructure of red cell membranes (“ghosts”), prepared by gradual osmotic hemolysis, was studied during storage in parallel with some of the biochemical changes that characterize the storage lesion. The loss of cholesterol and phospholipids and the decrease in DPG and the delayed decrease of ATP together with the increase in inorganic phosphate indicated a typical storage lesion, however, no significant modification in the proportion of structurally old red cell membranes was found. It is concluded that the storage lesion can not be morphologically identified with physiological aging of erythrocytes from the electron microscopical point of view.