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Maintenance of ATP Level of Incubated Human Red Cells by Controlling the p H
Author(s) -
Bishop Charles
Publication year - 1962
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1962.tb00265.x
Subject(s) - glycolysis , heparin , lactic acid , red blood cell , biochemistry , red cell , chemistry , adenosine triphosphate , titration , metabolism , biology , bacteria , medicine , inorganic chemistry , genetics
Fresh human blood was incubated at 37 C. in ACD solution, heparin‐glucose solution, and heparin‐glucose solution with p H controlled by automatic titration with sterile NaHCO 3 solution. In ACD solution glycolysis of the red cells slowed down with time and concomitantly the ATP level decreased sharply. In heparin‐glucose solution, glycolysis continued more vigorously and the ATP levels were maintained longer. In the flasks in which the lactic acid was neutralized continuously, glycolysis proceeded almost unabated, and the ATP levels were correspondingly well maintained. Red cell viability and survival are presently assumed to be related to glycolytic maintenance of adequate levels of ATP. These studies demonstrate that storage of blood in heparin‐glucose may prolong the red cell viability over that in ACD, and that neutralization of the lactic acid produced in glycolysis gives such dramatic improvement that a whole new system of blood storage may be predicated on this principle.