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Reaction of I 131 Anti‐Rh o (D) with Enzyme Treated Red Cells
Author(s) -
Masouredis S. P.
Publication year - 1962
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1962.tb00259.x
Subject(s) - enzyme , papain , proteolytic enzymes , trypsin , red cell , chemistry , hemolysis , antigen , microbiology and biotechnology , antibody , biochemistry , red blood cell , rh blood group system , biology , immunology , medicine
The effect of proteolytic enzymes (papain, trypsin, ficin and bromelin) on Rh o (D) positive and negative red cells was studied with I 131 anti‐Rh o (D). Enzymatic pretreatment of red cells results in almost a two‐fold increase in the amount of I 131 antiRh o (D) bound to these cells. The “enzyme exposed Rh o (D)” antigen by some criteria (stability to hemolysis, absence of an increase in negative cells and studies on the supernatant solutions) appears to have the same specificity as the Rh o (D) antigen on the untreated cell. The ability of proteolytic enzymes to produce this effect on Rh o (D) positive red cells does not correlate directly with the ability of these enzymes to digest casein, although the effect appears to be associated with the proteolytic activity of these enzymes. The amount of increase in uptake of I 131 anti‐Rh o (D) induced by enzymes is directly proportional to the quantity of antibody bound by the untreated cell, irrespective of the Rh phenotype or zygosity of the red cell.