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I. Analysis of the Immune Agglutination of Red Cells
Author(s) -
Goodman Harold S.
Publication year - 1962
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1962.tb00248.x
Subject(s) - agglutination (biology) , agglutinin , antigen , antibody , immune system , immunology , chemistry , biology , microbiology and biotechnology , lectin
An attempt has been made to clarify the basis of the agglutination reaction of red cells and to indicate how immunological factors such as antigen and antibody heterogeneity, number of antigenic sites and temperature influence this reaction. Experimentally the influence of antibody heterogeneity and temperature on the stability of the antigen‐antibody complex was illustrated by quantitatively comparing the agglutination obtained by two chromatographic fractions of a human anti‐A serum at three different temperatures. The influence of antigenic heterogeneity, and the number of antigenic sites per cell was illustrated by comparing antibody absorption by A 1 and A 2 cells at different temperatures. The agglutination reactions characteristic of the human A subgroups and the quantitation of agglutinin activity have been analyzed.