Premium
The Stability of Factor VIII (Antihemophilic Globulin) in Fresh‐Frozen Blood Bank Plasma
Author(s) -
Perkins Herbert A.,
Rolfs Mary R.,
Acra D. Jose
Publication year - 1962
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1962.tb00246.x
Subject(s) - blood collection , blood preservation , fresh frozen plasma , globulin , blood bank , medicine , chemistry , endocrinology , andrology , platelet , emergency medicine , medical emergency
Factor VIII (antihemophilic globulin) levels in normal donors varied between 50 and 200 per cent of the mean. The level of Factor VIII in stored fresh‐frozen plasma was directly related to the level of the donor and appears likely to be far less influenced by the duration of storage. Collection of blood in plastic bags with the aid of a rocking vacuum device and storage of the plasma at –28 C. for two months resulted in loss of only 10 per cent of Factor VIII activity. Results were definitely inferior when blood was collected by a simple gravity technic. Re‐exploration of factors previously shown to influence Factor VIII stability confirmed the fact that temperatures well below freezing and a low pH (such as occurs in ACD plasma) are essential to prevent loss of activity. It is concluded that plasmas with high levels of Factor VIII activity can be obtained best by selecting donors with known high concentrations of this factor.