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Effects of Salt Concentration, p H, and Certain Ions on the Agglutination of Human Erythrocytes by Isohemagglutinins
Author(s) -
Yokoyama M.,
Finlayson J. S.
Publication year - 1961
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1961.tb00035.x
Subject(s) - tris , agglutination (biology) , molar concentration , chemistry , titer , hydroxymethyl , tonicity , isotonic , ionic strength , chromatography , nuclear chemistry , biochemistry , stereochemistry , aqueous solution , immunology , medicine , antibody , biology , organic chemistry
The effect of molarity of NaCl, KC1, LiCl, tris(hydroxymethyl)aminomethane acetate, CaCl 2 , MgCl 2 , Na 2 SO 4 , K 2 SO 4 ) and MgSO 4 solutions on the agglutination of human erythrocytes by isohemagglutinins of the ABO series has been studied, as has that of p H in “tris acetate” buffer. In all cases the maximum agglutination titer occurred at the isotonic or a slightly hypertonic concentration and was generally the same as that determined by diluting the given cells and serum with isotonic saline. Moreover, all salts exerted quantitatively similar effects on agglutination, the titer decreasing regularly as the molarity increased from 0.1544‐308 to 1.0. Apparent variations in the effects of different classes of salts were induced by expressing salt concentration as ionic strength or normality rather than molarity. In tris acetate buffers of ionic strengths 0.154 (isotonic) and 0.308, the titer was constant in the p H range 5.5 to 7.5–8.0 and decreased at either extreme.