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Specificity of the Agglutinating Phase of Immunohematologic Reactions with Non‐Saline Agglutinating Antisera
Author(s) -
Prager Morton D.
Publication year - 1961
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1961.tb00033.x
Subject(s) - antiserum , antibody , antigen , saline , biology , homologous chromosome , chemistry , microbiology and biotechnology , virology , immunology , biochemistry , gene , endocrinology
When mixtures of stained and unstained human red cells of different antigenic composition are mixed with homologous non‐saline agglutinating antisera in a small Petri dish, two distinctly different clumps of cells are formed. This observation suggests that the agglutinating phase of the reaction is specific in accord with the Marrack hypothesis and further implies that these antibodies are not univalent but are bi‐ or polyvalent. As a result of formation of a single cell clump, application of this technic has led to the discovery of a serum containing a heretofore unrecognized anti‐CD antibody.