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Studies of Blood Group Antibodies
Author(s) -
Abelson Neva M.,
Rawson Arnold J.
Publication year - 1961
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1961.tb00022.x
Subject(s) - serology , globulin , antibody , gamma globulin , ultracentrifuge , chemistry , fraction (chemistry) , medicine , immunology , chromatography
Examples of blood group antibodies were subjected to density gradient ultracentrifugation and anion‐cation chromatography on modified cellulose adsorbents and three fractions were collected. The first fraction owed its serologic activity to the presence of γ2 globulins with a 7S sedimentation constant; the principal serologically‐active component in fraction two was γ1 globulin of the 7S class; in most cases the serologically‐active component of the third fraction was γ1 globulin of the 19S class. Two anti‐A donors were found to have most of the serologic activity in fraction three before injection of blood group substance and a marked rise in fractions two and three after stimulation. In anti‐M, anti‐P and saline anti‐D sera the serologic activity associated with S19 globulins equalled or exceeded the activity with the S7 globulins. Group O serums were studied before injection of A and B substances and S7 activity was preponderant in 48 per cent whereas post‐immunization it was preponderant in 88 per cent. Fraction one activity was also preponderant in examples of anti‐K, anti‐Fy a , anti‐s, anti‐Jk a and anti‐Good. Those antibodies which are most likely to cause erythroblastosis fetalis are those which are characterized by a predominance of the S7, γ2 component.