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Optimal Conditions for Storage of Fresh Frozen Plasma
Author(s) -
Anstall Harold B.,
GroveRasmussen Morten,
Shaw Robert S.
Publication year - 1961
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1111/j.1537-2995.1961.tb00018.x
Subject(s) - factor xi , chemistry , fresh frozen plasma , thromboplastin , clotting factor , coagulation , chromatography , zoology , medicine , platelet , biology
Small aliquots of plasma in 3.8 per cent trisodium citrate were stored at −15, −30 and −50 C. Lengthening of the recalcification time after 24 hours followed by progressive shortening for 10–14 days was observed at −15 and −50 C. At −30 C. no significant changes occurred until two months had elapsed. Assay of clotting factors VII and X, VIII and IX revealed essentially that the activity of each rose gradually over a span of 12–14 days after an initial decline at −15 C. and −50 C. At −30 C. factors VIII and IX were stable for at least two months. Factor V level declined gradually at −15 C. and −50 C. but no significant fall was observed at −30 C. The increase in the activity of factor VIII observed during storage at −15 and −50 C. was eliminated by prior adsorption with Celite which is believed to remove an activation product of Hageman factor and Plasma Thromboplastin Antecedent. There seemed to be somewhat greater instability of plasma stored in untreated glass containers than in cellulose nitrate or in siliconized glass. Storage temperatures below −40 C. and above −25 C. appear to be unfavorable for plasma coagulation factors.