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Evaluation of Steam Penetration and Sterilization of Natural Latex Wraps
Author(s) -
Rossanese Matteo,
Gasson James,
Barker Colin,
Bowlt Kelly
Publication year - 2014
Publication title -
veterinary surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 79
eISSN - 1532-950X
pISSN - 0161-3499
DOI - 10.1111/j.1532-950x.2014.12168.x
Subject(s) - sterilization (economics) , spore , medicine , agar , nutrient agar , ethylene oxide , dry heat , chromatography , geobacillus stearothermophilus , food science , surgery , microbiology and biotechnology , chemistry , biology , materials science , bacteria , biochemistry , composite material , polymer , genetics , copolymer , monetary economics , economics , foreign exchange market , foreign exchange , thermophile , enzyme
Objective To evaluate the efficacy of steam and ethylene oxide (EtO) sterilization of Vetrap™ bandages. Study Design Prospective experimental study. Sample Population Vetrap™ bandages (n = 70; 35 as supplied by the manufacturer, 35 unwound and tightly rewound). Methods Vetrap™ bandage rolls (n = 60) marked with a 1 cm square were inoculated with 0.1 mL Geobacillus stearothermophilus spores, packaged in a pouch together with independent sterilization indicators and assigned into 3 sub‐groups for sterilizer type: dynamic air removal, gravity displacement, and bench‐top pre‐vacuum and further sub‐divided into 2 sterilization temperatures. Vetrap™ bandages rolls (n = 10) were inoculated with 0.1 mL Bacillus atrophaeus spores in the same manner and underwent EtO sterilization. After sterilization, the 1 cm marked square was aseptically resected to the level of the cardboard tube and enriched in a flask containing 10 mL tryptic soy broth for 24 hours at 60°C for G. stearothermophilus and 37°C for B. atrophaeus . Aliquots were subsequently plated on a Petri dish of tryptic soy agar and incubated at 60°C for G. stearothermophilus and 37°C for B. atrophaeus for 24 hours. Samples were scored positive if colonies of indicator organism were present on the nutrient agar after 24 hours. Results Three Vetrap™ bandages yielded post‐sterilization growth of G. stearothermophilus : 2 from the dynamic air removal sterilizer at 134°C for 3.5 minutes, and 1 from the bench‐top pre‐vacuum sterilizer at 121°C for 15 minutes. After EtO sterilization, no positive samples were detected. Conclusions Steam sterilization may be incomplete for Vetrap™ bandages whereas EtO showed complete destruction of resistant bacterial spores.