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Cell Growth Characteristics and Differentiation Frequency of Adherent Equine Bone Marrow–Derived Mesenchymal Stromal Cells: Adipogenic and Osteogenic Capacity
Author(s) -
VIDAL MARTIN A.,
KILROY GAIL E.,
JOHNSON JILL R.,
LOPEZ MANDI J.,
MOORE RUSTIN M.,
GIMBLE JEFFREY M.
Publication year - 2006
Publication title -
veterinary surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 79
eISSN - 1532-950X
pISSN - 0161-3499
DOI - 10.1111/j.1532-950x.2006.00197.x
Subject(s) - mesenchymal stem cell , adipogenesis , stromal cell , bone marrow , in vitro , medicine , andrology , cellular differentiation , stem cell , immunology , microbiology and biotechnology , biology , pathology , biochemistry , gene
Objectives— To characterize equine bone marrow (BM)‐derived mesenchymal stem cell (MSC) growth characteristics and frequency as well as their adipogenic and osteogenic differentiation potential. Study Design— In vitro experimental study. Animals— Foals (n=3, age range, 17–51 days) and young horses (n=5, age range, 9 months to 5 years). Methods— Equine MSCs were harvested and isolated from sternal BM aspirates and grown up to passage 10 to determine cell‐doubling (CD) characteristics. Limit dilution assays were performed on primary and passaged MSCs to determine the frequency of colony‐forming units with a fibroblastic phenotype (CFU‐F), and the frequency of MSC differentiation into adipocytes (CFU‐Ad) and osteoblasts (CFU‐Ob). Results— Initial MSC isolates had a lag phase with a significantly longer CD time (DT=4.9±1.6 days) compared with the average DT (1.4±0.22 days) of subsequent MSC passages. Approximately 1 in 4224±3265 of the total nucleated BM cells displayed fibroblast colony‐forming activity. Primary MSCs differentiated in response to adipogenic and osteogenic inductive conditions and maintained their differentiation potential during subsequent passages. Conclusions— The frequency, in vitro growth rate, and adipogenic and osteogenic differentiation potential of foals and young adult horses are similar to those documented for BM MSCs of other mammalian species. Clinical Relevance— The results have direct relevance to the use of BM as a potential source of adult stem cells for tissue engineering applications in equine veterinary medicine.