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Effects of Ovariohysterectomy on Canine Blood Neutrophil Respiratory Burst: a Chemiluminescence Study
Author(s) -
Väisänen Misse,
Lilius EsaMatti,
Mustonen Leena,
Raekallio Marja,
Kuusela Erja,
Koivisto Maarit,
Vainio Outi
Publication year - 2004
Publication title -
veterinary surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 79
eISSN - 1532-950X
pISSN - 0161-3499
DOI - 10.1111/j.1532-950x.2004.04077.x
Subject(s) - medicine , respiratory burst , absolute neutrophil count , clinical significance , zymosan , neutrophile , perioperative , acepromazine , chemiluminescence , anesthesia , respiratory system , complete blood count , butorphanol , whole blood , medetomidine , immunology , inflammation , neutropenia , blood pressure , chemotherapy , heart rate , in vitro , biochemistry , chemistry , organic chemistry
Objective— To examine blood neutrophil counts and luminol‐enhanced chemiluminescence (CL) responses in dogs undergoing ovariohysterectomy (OH), premedicated with 2 different drugs. Study Design— Randomized clinical study. Animals— Forty‐two healthy client‐owned bitches. Methods— Dogs had OH under isoflurane anesthesia with either acepromazine or medetomidine, both in combination with butorphanol, administered as preanesthetic medication. Blood samples were collected when the dog was admitted, at the end of surgery, and the next day (approximately 20 hours after surgery). Blood neutrophils were counted automatically, and neutrophil oxidative activity was assessed by measuring blood CL responses (induced by opsonized zymosan and enhanced by luminol) at 37°C for 40 minutes. Results— Number of circulating neutrophils was significantly increased the day after surgery reflected by enhanced blood CL responses. Neutrophil CL, however, was not significantly altered. No significant differences were detected for perioperative Polymorphonuclear neutrophil (PMN) characteristics between the 2 preanesthetic regimens. Conclusions— In conclusion, despite clearly increasing the number of circulating neutrophils, OH did not significantly affect neutrophil respiratory burst, as measured by whole‐blood CL responses. Clinical Relevance— Surgical operation of moderate intensity (e.g., OH) did not significantly alter one of the important immune functions, neutrophil oxidative activity. Further studies are warranted to confirm the significance of this finding, and to assess the value of following this variable in different animal patient populations.