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Effects of Perioperative Granulocyte Colony‐Stimulating Factor on Horses With Ascending Colonic Ischemia
Author(s) -
SULLIVAN KENT E.,
SNYDER JACK R.,
MADIGAN JOHN E.,
PASCOE JOHN R.,
FARVER THOMAS B.,
THURMOND MARK C.,
ANDRESEN JEFF W.
Publication year - 1993
Publication title -
veterinary surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 79
eISSN - 1532-950X
pISSN - 0161-3499
DOI - 10.1111/j.1532-950x.1993.tb00410.x
Subject(s) - medicine , granulocyte colony stimulating factor , peripheral blood mononuclear cell , granulocyte , neutrophilia , antimicrobial , hematopoietic growth factor , white blood cell , penicillin , antibiotics , anesthesia , pharmacology , immunology , haematopoiesis , chemotherapy , stem cell , microbiology and biotechnology , biochemistry , chemistry , genetics , in vitro , biology
Granulocyte colony‐stimulating factor (G‐CSF) is a glycoprotein that regulates the proliferation and maturation of hematopoietic progenitor cells and modulates the function of mature neu‐trophils. The responses to administration of G‐CSF alone, and in combination with antimicrobials, were studied in an equine model of ascending colon ischemia. Complete segmental colonic ischemia (3.75 hours) with pelvic flexure enterotomy was created in four treatment groups. Group 1 horses received recombinant canine G‐CSF (10 μg/kg, every 24 hours, intramuscularly), gentamicin sulfate (2.2 mg/kg, every 8 hours, intravenously), and potassium penicillin G (40,000 lU/kg, every 6 hours, intravenously). Group 2 horses were treated with the G‐CSF vehicle and antimicrobials as for group 1. Group 3 horses received G‐CSF and the antimicrobial drug vehicles, and group 4 horses served as the untreated control receiving G‐CSF vehicle and antimicrobial vehicles. The results for 20 horses, five horses in each group, were compared. Treatment with G‐CSF was associated with an increased concentration of white blood cells, band neutrophils, neutrophils, lymphocytes, and monocytes in the peripheral blood after surgery. Antimicrobial administration had no detectable effect on cell concentrations after surgery. Administration of G‐CSF was associated with an increased concentration of nucleated cells in the peritoneal fluid including neutrophils, small mononuclear cells and large mononuclear cells. Horses that developed incisional infections had lower neutrophil concentrations in the peripheral blood on postoperative day 2 than horses without infected incisions. These results suggested that the prophylactic administration of G‐CSF may be useful in the treatment of patients at risk for developing neutropenia after surgery.