Premium
Regional Limb Perfusion for Antibiotic Treatment of Experimentally Induced Septic Arthritis
Author(s) -
WHITEHAIR KAREN J.,
BOWERSOCK TERRY L.,
BLEVINS WILLIAM E.,
FESSLER JOHN F.,
WHITE MELVIN R.,
SICKLE DAVID C.
Publication year - 1992
Publication title -
veterinary surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 79
eISSN - 1532-950X
pISSN - 0161-3499
DOI - 10.1111/j.1532-950x.1992.tb01713.x
Subject(s) - gentamicin sulfate , gentamicin , medicine , synovial fluid , septic arthritis , perfusion , staphylococcus aureus , antibiotics , arthritis , synovial membrane , surgery , pathology , microbiology and biotechnology , bacteria , osteoarthritis , genetics , alternative medicine , biology
Septic arthritis was induced in one antebrachiocarpal joint of seven horses by the intraarticular injection of 1 mL Staphylococcus aureus suspension containing a mean of 10 5 colony‐forming units. Twenty‐four hours after inoculation, four horses were treated by regional perfusion with 1 g of gentamicin sulfate, and three horses received 2.2 mg/kg gentamicin sulfate intravenously (IV) every 6 hours. Synovial fluid was collected for culture and cytology at regular intervals, and the synovial membranes were collected for culture and histologic examination at euthanasia 24 hours after the first treatment. Gentamicin concentration in the septic synovial fluid after three successful perfusions was 221.2 ± 71.4 (SD) μg/mL; after gentamicin IV, it was 7.6 ± 1.6 (SD) μg/mL. The mean leukocyte count in the inoculated joints decreased significantly by hour 24 in the successfully perfused joints. Terminal bacterial cultures of synovial fluid and synovial membranes were negative in two horses with successfully perfused joints. S. aureus was isolated from the infected joints in all three horses treated with gentamicin IV.