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Effect of Three Methods for Cleaning Dentures on Biofilms Formed In Vitro on Acrylic Resin
Author(s) -
Oliveira Paranhos Helena F.,
SilvaLovato Cláudia H.,
De Souza Raphael F.,
Cruz Patricia C.,
De FreitasPontes Karina M.,
Watanabe Evandro,
Ito Izabel Y.
Publication year - 2009
Publication title -
journal of prosthodontics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.902
H-Index - 60
eISSN - 1532-849X
pISSN - 1059-941X
DOI - 10.1111/j.1532-849x.2009.00450.x
Subject(s) - streptococcus mutans , biofilm , acrylic resin , microbiology and biotechnology , candida albicans , distilled water , chemistry , staphylococcus aureus , candida tropicalis , dentures , corpus albicans , antimicrobial , food science , dentistry , bacteria , biology , chromatography , medicine , genetics , organic chemistry , coating
Purpose: The aim of this study was to evaluate the effect of three denture hygiene methods against different microbial biofilms formed on acrylic resin specimens. Materials and methods: The set (sterile stainless steel basket and specimens) was contaminated (37°C for 48 hours) by a microbial inoculum with 10 6 colony‐forming units (CFU)/ml (standard strains: Staphylococcus aureus , Streptococcus mutans , Escherichia coli, Candida albicans, Pseudomonas aeruginosa , and Enterococcus faecalis ; field strains: S. mutans , C. albicans , C. glabrata , and C. tropicalis ). After inoculation, specimens were cleansed by the following methods: (1) chemical: immersion in an alkaline peroxide solution (Bonyplus tablets) for 5 minutes; (2) mechanical: brushing with a dentifrice for removable prostheses (Dentu Creme) for 20 seconds; and (3) a combination of chemical and mechanical methods. Specimens were applied onto a Petri plate with appropriate culture medium for 10 minutes. Afterward, the specimens were removed and the plates incubated at 37°C for 48 hours. Results: Chemical, mechanical, and combination methods showed no significant difference in the reduction of CFU for S. aureus , S. mutans (ATCC and field strain), and P. aeruginosa . Mechanical and combination methods were similar and more effective than the chemical method for E. faecalis , C. albicans (ATCC and field strain), and C. glabrata . The combination method was better than the chemical method for E. coli and C. tropicalis , and the mechanical method showed intermediate results. Conclusion: The three denture hygiene methods showed different effects depending on the type of microbial biofilms formed on acrylic base resin specimens.