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Effect of Different Exposure Times on Microwave Irradiation on the Disinfection of a Hard Chairside Reline Resin
Author(s) -
Mima Ewerton G.,
Pavarina Ana Claudia,
Neppelenbroek Karin Hermana,
Vergani Carlos Eduardo,
Spolidorio Denise Madalena P.,
Machado Ana Lucia
Publication year - 2008
Publication title -
journal of prosthodontics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.902
H-Index - 60
eISSN - 1532-849X
pISSN - 1059-941X
DOI - 10.1111/j.1532-849x.2007.00277.x
Subject(s) - sterilization (economics) , serial dilution , irradiation , microwave irradiation , microwave oven , contamination , saline , candida albicans , incubation , inoculation , microbiology and biotechnology , chemistry , materials science , biology , medicine , microwave , horticulture , pathology , alternative medicine , monetary economics , foreign exchange market , endocrinology , ecology , biochemistry , quantum mechanics , nuclear physics , catalysis , foreign exchange , physics , economics
Purpose: This study evaluated the effectiveness of different exposure times of microwave irradiation on the disinfection of a hard chairside reline resin. Materials and Methods: Sterile specimens were individually inoculated with one of the tested microorganisms ( Pseudomonas aeruginosa , Staphylococcus aureus , Candida albicans , and Bacillus subtilis ) and incubated for 24 hours at 37°C. For each microorganism, 10 specimens were not microwaved (control), and 50 specimens were microwaved. Control specimens were individually immersed in sterile saline, and replicate aliquots of serial dilutions were plated on selective media appropriate for each organism. Irradiated specimens were immersed in water and microwaved at 650 W for 1, 2, 3, 4, or 5 minutes before serial dilutions and platings. After 48 hours of incubation, colonies on plates were counted. Irradiated specimens were also incubated for 7 days. Some specimens were prepared for scanning electron microscopic (SEM) analysis. Results: Specimens irradiated for 3, 4, and 5 minutes showed sterilization. After 2 minutes of irradiation, specimens inoculated with C. albicans were sterilized, whereas those inoculated with bacteria were disinfected. One minute of irradiation resulted in growth of all microorganisms. SEM examination indicated alteration in cell morphology of sterilized specimens. The effectiveness of microwave irradiation was improved as the exposure time increased. Conclusion: This study suggests that 3 minutes of microwave irradiation can be used for acrylic resin sterilization, thus preventing cross‐contamination.