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Histomorphologic Characterization of Noncarious and Caries‐Affected Dentin/Adhesive Interfaces
Author(s) -
HajAli Reem,
Walker Mary,
Williams Karen,
Wang Yong,
Spencer Paulette
Publication year - 2006
Publication title -
journal of prosthodontics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.902
H-Index - 60
eISSN - 1532-849X
pISSN - 1059-941X
DOI - 10.1111/j.1532-849x.2006.00079.x
Subject(s) - dentin , adhesive , molar , dentistry , materials science , penetration (warfare) , composite material , medicine , layer (electronics) , operations research , engineering
Purpose: The purpose of this study was to compare the dentin/adhesive interfacial characteristics when bonding to noncarious as well as caries‐affected dentin.Materials and Methods: Seven extracted, unerupted, third molars were sectioned into halves. Artificial caries was created on one‐half of each tooth, leaving the other half as a control. Dentin surfaces were treated with UNO adhesive according to the manufacturer's instructions for the wet‐bonding technique and under environmental conditions present in the oral cavity. Dentin/adhesive interface sections of each half‐tooth were stained with Goldner's trichrome, a classic bone stain, and examined using light microscopy. The width of exposed collagen was measured directly from photomicrographs, and adhesive penetration was analyzed qualitatively.Results:The degree and extent to which the adhesive encapsulated the demineralized dentin matrix were reflected in the color difference in the stained sections with the noncarious dentin sections showing a degree of collagen encapsulation superior to that of the caries‐affected dentin sections. The overall mean widths of exposed collagen were significantly ( p ≤ .05) greater at the caries‐affected dentin/adhesive interface, 8.6 (1.7) μm, as compared with those at the noncarious dentin/adhesive interface, 6.0 (1.5) μm .Conclusions:The morphologic characteristics of the caries‐affected dentin/interface suggest an increase in the exposed collagen zone and a decrease in the quality of the adhesive infiltration when compared with noncarious dentin. The evidence suggests that dentin substrate characteristics have a significant effect on the dentin/adhesive interface structure.