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Age‐ and Ethanol Concentration‐Dependent Effects of Acute Binge Drinking in the HIV ‐1 Transgenic Rat
Author(s) -
Sarkar Sraboni,
Mao Xin,
Liu Chuang,
Chang Sulie L.
Publication year - 2013
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.2012.01860.x
Subject(s) - ethanol , alcohol , binge drinking , spleen , medicine , endocrinology , population , chemistry , pharmacology , biochemistry , environmental health , alcohol consumption
Background Binge drinking is common in young people. Alcoholic beverages vary significantly in their ethanol ( E t OH ) concentration (alcohol by volume). We previously showed E t OH concentration‐dependent activation of the hypothalamic supraoptic nucleus. In the HIV ‐infected population, incidence of alcohol abuse is close to 50%. We found age‐dependent expression of HIV ‐1 viral proteins in the HIV ‐1 transgenic ( HIV ‐1 T g) rat. Thus, we hypothesized that there are age‐ and E t OH concentration‐dependent effects of binge drinking in HIV ‐1‐positive individuals. Methods Blood ethanol concentration was measured in adult F 344 rats after gavage (i.g.) administration of water, 20% E t OH , or 52% E t OH . We also compared expression of the HIV ‐1 viral protein T at in the brain, spleen, and liver of adult and adolescent HIV ‐1 T g rats following binge i.g. administration of water, 20% E t OH , or 52% E t OH for 3 days (4.8 g/kg/d) using absolute quantitative real‐time reverse transcription‐polymerase chain reaction. In a parallel study, we assessed age‐dependent motor function in the HIV ‐1 T g rats 1 day after exposure to 20% E t OH using the open‐field test. Results Blood ethanol concentration was significantly higher in the 52% E t OH ‐treated F 344 rats compared to the 20% E t OH animals at 90 minutes posttreatment. In the adult HIV ‐1 T g rats, HIV ‐1 T at expression (copies per microgram of total RNA ) was significantly increased in the brain, liver, and spleen of the 52% E t OH group, but not in the 20% E t OH group. However, in the adolescent animals, HIV ‐1 T at expression in the 52% E t OH group was increased in the brain and liver, but not in the spleen. A significant reduction in locomotor activity occurred in 20% E t OH ‐treated adult HIV ‐1 T g rats compared to the water control, although no difference was observed in the adolescent HIV ‐1 T g animals. Conclusions Our data indicate that binge alcohol drinking can have age‐ and E t OH concentration‐dependent effects in the presence of HIV ‐1 infection.