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Selenium or Selenium Plus Folic Acid–Supplemented Diets Ameliorate Renal Oxidation in Ethanol‐Exposed Pups
Author(s) -
Ojeda Maria Luisa,
Nogales Fatima,
Murillo Maria Luisa,
Carreras Olimpia
Publication year - 2012
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.2012.01788.x
Subject(s) - lipid peroxidation , chemistry , lactation , endocrinology , antioxidant , medicine , oxidative stress , kidney , offspring , superoxide dismutase , selenium , catalase , low protein diet , biochemistry , biology , pregnancy , organic chemistry , genetics
Background Ethanol (EtOH) exposure during gestation and lactation induces an oxidative stress in offspring. In kidney, the oxidative damage is the primary pathway to alcohol‐induced injury. In this study, we have demonstrated that a diet supplemented with selenium (Se) (0.5 ppm) or with Se (0.5 ppm) + folic acid (8 ppm) administered to EtOH‐exposed (20% v/v) dams during gestation and lactation prevents the oxidative EtOH‐provoked effects in their offspring's kidneys. Methods All the studies were performed on 21‐day‐old pups. Serum, urine, and kidney Se levels were assessed by graphite‐furnace atomic absorption spectrometry. Se and creatinine clearance, antioxidant enzyme activities, and lipid and protein peroxidation were determined by a spectrophotometric method in kidney. Results Dietary supplementation treatments used could not improve the glomerular filtration function altered by EtOH exposure during gestation and lactation; however, they did improve renal Se deposits, renal development, and renal protein content while decreasing lipid and protein oxidation and modifying antioxidant enzymes' activity. Conclusions Se or Se + folic acid supplementations improve renal development and protein content and modify antioxidant enzymes' activity, decreasing lipid and protein oxidation after EtOH exposure. In this context, a double‐supplemented diet appears to reduce protein peroxidation more efficiently than the Se‐only‐supplemented one, probably via superoxide dismutase and catalase.