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Ethanol Drinking Microstructure of a High Drinking in the Dark Selected Mouse Line
Author(s) -
BarkleyLevenson Amanda M.,
Crabbe John C.
Publication year - 2012
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.2012.01749.x
Subject(s) - preference test , zoology , ethanol , microstructure , chemistry , endocrinology , biology , biochemistry , preference , mathematics , statistics , crystallography
Background The High Drinking in the Dark ( HDID ) selected mouse line was bred for high blood ethanol ( E t OH ) concentration ( BEC ) following the limited access drinking in the dark ( DID ) test and is a genetic animal model of binge‐like drinking. This study examines the microstructure of E t OH drinking in these mice and their control line during 3 versions of the DID test to determine how drinking structure differences might relate to overall intake and BEC . Methods Male mice from the HDID ‐1 replicate line and HS / N pt progenitor stock were tested in separate experiments on 2‐ and 4‐day versions of the DID test, and on a 2‐day 2‐bottle choice DID test with 20% E t OH and water. Testing took place in home cages connected to a continuous fluid intake monitoring system, and drinking during the DID test was analyzed for drinking microstructure. Results HDID‐1 mice had more drinking bouts, shorter interbout interval, larger bout size, greater total E t OH intake, and higher BEC s than HS / N pt mice on the second day of the 2‐day DID test. The 4‐day DID test showed greater bout size, total E t OH intake, and BEC in the HDID ‐1 mice than the HS / N pt mice. Total E t OH intake and BEC s for the HDID ‐1 mice in the DID tests averaged 2.6 to 3.0 g/kg and 0.4 to 0.5 mg/ml, respectively. The 2‐bottle choice test showed no genotype differences in drinking microstructure or total consumption but did show greater preference for the E t OH solution in HDID ‐1 mice than HS / Npt . Conclusions These results suggest that inherent differences in E t OH drinking structure between the HDID ‐1 and HS / N pt mice, especially the larger bout size in the HDID ‐1 mice, contribute to the difference in intake during the standard DID test.