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Actin Dynamics in Development of Behavioral Sensitization After Withdrawal from Long‐Term Ethanol Administration to Mice
Author(s) -
Shibasaki Masahiro,
Kurokawa Kazuhiro,
Mizuno Koji,
Suzuki Tsutomu,
Ohkuma Seitaro
Publication year - 2012
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.2012.01747.x
Subject(s) - sensitization , inhalation , chemistry , ethanol , medicine , endocrinology , anesthesia , biochemistry , immunology
Background and Methods The present study investigated the role of actin depolymerizing factor ( ADF ) in the brain of mice after withdrawal from continuous ethanol ( E t OH ) vapor inhalation for 9 days using C 57 BL /6 J and ADF mutant mice. Results C57BL/6J mice with withdrawal signs 10 hours after withdrawal from E t OH vapor inhalation showed transient and significant enhancement of locomotor activity by a single injection of E t OH (2 g/kg, i.p.) and of E t OH ‐induced place preference 3 days after withdrawal from E t OH vapor inhalation, suggesting the development of sensitization of locomotion activity to E t OH and of place preference 3 days after withdrawal from E t OH in C57BL/6J mice with E t OH physical dependence. The levels of ADF and G ‐actin in the ventral tegmental area, including a little bit of surrounding tissues, increased immediately (0 hours), 10 hours, and 3 days after withdrawal from E t OH vapor. F ‐actin, synaptic vesicle‐associated protein 38, and postsynaptic density 95 increased 0 hours and 3 days after withdrawal with their decreases 10 hours after withdrawal from E t OH vapor. An F ‐actin stabilizing agent phalloidin (3 nmol/mouse/d, i.c.v., once a day) administered daily for 3 days after withdrawal from continuous E t OH vapor inhalation for 9 days significantly suppressed the increase in both E t OH ‐induced place preference and locomotor activity by a single injection of E t OH 3 days after withdrawal from long‐term E t OH vapor inhalation for 9 days. In addition, the changes in behavioral sensitization in ADF mutant mice were significantly weaker than those observed in C57BL/6J mice (wild‐type mice for ADF mutant mice). Conclusions The findings presented here suggest that withdrawal from E t OH physical dependence causes behavioral sensitization to E t OH , which may be, at least in part, mediated by alternation of actin dynamics.