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Detection of Biomarkers for Alcoholism by Two‐Dimensional Differential Gel Electrophoresis
Author(s) -
Wu Di,
Tomonaga Takeshi,
Sogawa Kazuyuki,
Satoh Mamoru,
Sunaga Masahiko,
Nezu Masahiko,
OhIshi Masamichi,
Kodera Yoshio,
Maeda Tadakazu,
Ochiai Takenori,
Nomura Fumio
Publication year - 2007
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.2006.00289.x
Subject(s) - carbohydrate deficient transferrin , transferrin , abstinence , albumin , gel electrophoresis , blood proteins , agarose gel electrophoresis , electrophoresis , differential diagnosis , microbiology and biotechnology , medicine , chemistry , biology , pathology , chromatography , biochemistry , gene , psychiatry , alcohol , alcohol consumption
Background: Up to now, γ ‐glutamyltransferase ( γ ‐GTP) and carbohydrate‐deficient transferrin (CDT) have been used as markers for alcoholism most widely, but they are not satisfactory regarding sensitivity/specificity. Therefore, for novel markers need to be searched. Methods: To detect new biomarkers for alcoholism, albumin and immunoglobulinG were first removed from serum. Then, protein profiles of 12 serum samples before and after 3 months of abstinence treatment were examined using agarose 2‐dimensional differential gel electrophoresis (agarose2‐D DIGE). Two‐dimensional differential gel electrophoresis images were analyzed using Shimadzu 2‐D Evolution Software. Results: Eight spots whose expression were significantly altered after abstinence were detected. Of these, 2 proteins increased and 6 proteins decreased after treatment. Conclusions: Altered expressions of several serum proteins after abstinence therapy were detected. They are promising markers for clinical application of alcoholism.