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Rapid Induction of Apoptosis in Gastrulating Mouse Embryos by Ethanol and Its Prevention by HB‐EGF
Author(s) -
Kilburn Brian A.,
Chiang Po Jen,
Wang Jun,
Flentke George R.,
Smith Susan M.,
Armant D. Randall
Publication year - 2006
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.2006.00008.x
Subject(s) - apoptosis , tunel assay , andrology , biology , annexin , programmed cell death , microbiology and biotechnology , terminal deoxynucleotidyl transferase , epidermal growth factor , dna fragmentation , embryo , endocrinology , biochemistry , receptor , medicine
Background: Ethanol exposure during gastrulation and early neurulation induces apoptosis within certain embryonic cell populations, leading to craniofacial and neurological defects. There is currently little information about the initial kinetics of ethanol‐induced apoptosis, and interest in the ability of endogenous survival factors to moderate apoptosis is growing. Ethanol alters intracellular signaling, leading to cell death in chick embryos, suggesting that apoptosis could occur rapidly and that signaling pathways activated by survival factors might reduce apoptosis. Methods: Pregnant mice were intubated with 1, 2, or 4 g/kg ethanol on day 7.5 of embryogenesis (E7.5) 1, 3, or 6, hours before harvesting gastrulation‐stage embryos. Control animals received maltose/dextran. Blood alcohol concentrations (BAC) were determined by gas chromatography. E7.5 embryos isolated from untreated dams were cultured in vitro for 1 or 3 hr with 0 or 400 mg% ethanol and 0 or 5 nM heparin‐binding epidermal growth factor (EGF)‐like growth factor (HB‐EGF). Apoptosis was quantified using fluorescence microscopy to detect annexin V binding and DNA fragmentation [ t erminal deoxynucleotidyl transferase‐mediated d U TP‐X n ick e nd l abeling (TUNEL)] in whole‐mount or sectioned embryos. Results: Both annexin V binding and TUNEL were elevated ( p <0.05) in embryos exposed in utero to 1 g/kg ethanol for 3 hours, increasing linearly with time and ethanol concentration. Apoptosis increased ( p <0.05) in all germ cell layers. Mice treated with 4 g/kg sustained BAC of 400 mg% for nearly 3 hours, significantly increasing apoptosis within the first hour. Cultured embryos exposed to 400 mg% ethanol displayed 2‐ to 3‐fold more TUNEL than vehicle‐treated embryos ( p <0.05); however, exogenous HB‐EGF prevented apoptosis. Conclusions: Ethanol rapidly produced apoptosis in gastrulation‐stage embryos, consistent with induction by intracellular signaling. The ethanol‐induced apoptotic pathway was blocked by the endogenous survival factor, HB‐EGF. Differences in the expression of survival factors within individual embryos could be partly responsible for variations in the teratogenic effects of ethanol among offspring exposed prenatally.