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Estriol Enhances Lipopolysaccharide‐Induced Increases in Nitric Oxide Production by Kupffer Cells via Mechanisms Dependent on Endotoxin
Author(s) -
Enomoto Nobuyuki,
Takei Yoshiyuki,
Kitamura Tsuneo,
Hirose Miyoko,
Ikejima Kenichi,
Sato Nobuhiro
Publication year - 2002
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.2002.tb02705.x
Subject(s) - estriol , lipopolysaccharide , kupffer cell , nitric oxide , neomycin , polymyxin b , medicine , endocrinology , chemistry , antibiotics , biochemistry , hormone
Background Estriol causes sensitization of Kupffer cells to lipopolysaccharide (LPS) via mechanisms dependent on gut‐derived LPS. Accordingly, this study examines the effect of estriol treatment on nitric oxide (NO) production from Kupffer cells. Methods Rats were given estriol (20 mg/kg body weight) intraperitoneally, and Kupffer cells were isolated 24 hr later. Some rats were treated for 4 days with 150 mg/kg/day of polymyxin B and 450 mg/kg/day of neomycin to prevent growth of intestinal bacteria, the primary source of endotoxin in the gastrointestinal tract. After addition of LPS, NO production by Kupffer cell was detected using a fluorescence indicator, DAF‐2. Results Twenty‐four hours after estriol administration, LPS‐induced NO production by Kupffer cells was enhanced as compared with control Kupffer cells. Sterilization of the gut with antibiotics blocked this enhancement. Conclusions Estriol treatment in vivo enhances LPS‐induced NO production in Kupffer cells.