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Differential Effects of T‐Cell Activation on Gastric and Small Bowel Permeability in Alcohol‐Consuming Mice
Author(s) -
Alnadjim Ziad,
Kayali Zeid,
Haddad Wael,
Holmes Earl W.,
Keshavarzian Ali,
Mittal Navdha,
Ivancic David,
Koehler Ronald,
Goldsmith David,
Waltenbaugh Carl,
Barrett Terrence A.
Publication year - 2002
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.2002.tb02689.x
Subject(s) - intestinal permeability , medicine , endocrinology , chemistry , tumor necrosis factor alpha , small intestine , cytokine , permeability (electromagnetism) , mannitol , interferon gamma , immunology , biology , biochemistry , membrane
Background A number of variables influence the effect(s) of alcohol on distinct segments of the intestine. In these studies, we examined the effect of T‐cell activation on gastric and small bowel permeability in alcohol‐fed mice. Methods Gastric permeability was assessed using sucrose absorption, whereas small bowel permeability was followed using the ratio of lactulose to mannitol absorption and inulin absorption. T cells were activated by injecting antigen OVA 323–339 into DO11.10 T‐cell receptor transgenic mice. Results T‐cell activation increased gastric and small bowel permeability through a pathway mediated by interferon‐γ and tumor necrosis factor. In mice that were fed a liquid diet that contained 30% ethanol‐derived calories for 2 weeks, T‐cell activation increased gastric permeability to levels greater than that observed in solid diet or pair‐fed, liquid control diet. By comparison, changes in small bowel permeability induced by T‐cell activation were abrogated in alcohol‐fed mice. Analysis of intestinal cytokine mRNA levels (interferon‐γ and tumor necrosis factor) indicated that relevant mucosal T‐cell function was preserved in alcohol‐fed mice Conclusions Overall, these data suggest that alcohol potentiates the effects of T‐cell activation on gastric permeability, at the same time blunting effects on small bowel permeability