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Beer Congener Stimulates Gastrointestinal Motility Via the Muscarinic Acetylcholine Receptors
Author(s) -
Fujii Wataru,
Hori Hisako,
Yokoo Yoshiaki,
Suwa Yoshihide,
Nukaya Haruo,
Taniyama Kohtaro
Publication year - 2002
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.2002.tb02590.x
Subject(s) - congener , gastric emptying , chemistry , muscarinic acetylcholine receptor , muscarinic acetylcholine receptor m3 , pharmacology , receptor antagonist , endocrinology , motility , receptor , antagonist , medicine , biochemistry , biology , stomach , chromatography , genetics
Background: Ethanol and alcoholic beverages are known to affect upper gastrointestinal motility in humans. Beer has been reported to accelerate gastric emptying compared with other beverages that contain the same ethanol concentrations. In this study, we investigated the mechanism that underlies the effects of beer congener on gastrointestinal motility. Methods: Gastric emptying activity was measured by means of movement of a semisolid test meal (0.05% phenol red/1.5% methylcellulose) in mice. To elucidate the mechanism for the effect of beer congener on gastrointestinal motility, we conducted receptor binding assays and contraction study by using longitudinal muscle from guinea pig ileum. Results: Beer congener (1 g/kg orally) enhanced gastric emptying of a semisolid meal in mice. The receptor binding assay revealed that beer congener bound to dopamine D 2 receptor and 5‐hydroxytryptamine (5‐HT) 3 receptor. These IC 50 values were more than 5 mg/ml. However, beer congener bound to 5‐HT 2 receptor, 5‐HT 4 receptor, and muscarinic M 3 receptor with IC 50 values of 2, 0.9, and 2 mg/ml, respectively. Beer congener (0.05–2 mg/ml) induced the contraction of longitudinal muscle from guinea pig ileum in a dose‐dependent manner. This effect was not affected by either tetrodotoxin (10 −6 M) or ketanserin (10 −7 ‐10 −5 M), an antagonist for the 5‐HT 2 receptor. On the other hand, 4‐DAMP (10 −8 −10 −5 M), an antagonist for the muscarinic M 3 receptor, inhibited the contraction of the longitudinal muscle induced by beer congener (2 mg/ml) dose dependently. Conclusions: Beer congener stimulates gastrointestinal motility via the muscarinic M 3 receptor.