Premium
Polymorphism of Tumor Necrosis Factor‐β and Alcohol Dehydrogenase Genes and Alcoholic Brain Atrophy in Japanese Patients
Author(s) -
Yamauchi Masayoshi,
Takamatsu Masashi,
Maezawa Yoshihiko,
Takagi Masaru,
Araki Takashi,
Satoh Shunnya,
Toda Gotaro,
Saito Saburo
Publication year - 2001
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.2001.tb02409.x
Subject(s) - atrophy , genotype , allele , medicine , polymorphism (computer science) , pathology , biology , gene , genetics
Background: Alcohol abuse can induce brain atrophy, but it only occurs in some alcoholics. Many inflammatory cytokines such as tumor necrosis factor (TNF) are produced rapidly in the brain by experimental or clinical injury. Method: To investigate whether genetic polymorphism of TNF was related to alcoholic brain atrophy, we determined restriction fragment‐length polymorphisms of the TNF‐β genes in 72 male alcoholics. Computed tomography was used to determine the severity of brain atrophy. Results: Digestion with Nco I and Msp I after polymerase chain reaction amplification showed that the TNFB 1 allele frequency was significantly higher in patients with brain atrophy than in those without brain atrophy (χ 2 = 10.20, p = 0.0034). A multivariate analysis that included age, total alcohol intake, ADH2 genotype, and TNF‐β genotype showed that the ADH2 1 /2 1 genotype and TNFB 1 /B 1 genotype are independently associated with alcoholic brain atrophy. These findings suggest that the TNFB 1 allele may be associated with alcoholic brain atrophy.