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Does Acetaldehyde Mediate Ethanol Action in the Central Nervous System?
Author(s) -
Mascia Maria Paola,
Maiya Rajani,
Borghese Cecilia M.,
Lobo Ingrid A.,
Hara Koji,
Yamakura Tomohiro,
Gong Diane H.,
Beckstead Michael J.
Publication year - 2001
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.2001.tb02163.x
Subject(s) - acetaldehyde , chemistry , ampa receptor , xenopus , nicotinic agonist , biophysics , ion channel , ligand gated ion channel , biochemistry , glutamate receptor , dopamine , inward rectifier potassium ion channel , g protein coupled inwardly rectifying potassium channel , receptor , acetylcholine receptor , ethanol , biology , endocrinology , g protein , gene
Background: Some of the effects of ethanol in the central nervous system are due to changes in function of ligand‐gated ion channels. Production of detectable amounts of acetaldehyde, a primary metabolite of ethanol, has been demonstrated in brain homogenates. The aim of this study was to determine whether central actions that are often attributed to ethanol may actually be mediated by acetaldehyde. Methods: The effects of acetaldehyde (1–1000 μM) were tested by two‐electrode voltage‐clamp electrophysiology in Xenopus laevis oocytes expressing 10 different ligand‐gated ion channel receptors [α 1 glycine; α 1 β 2 γ 2S γ‐aminobutyric acid (GABA) A ; ρ 1 GABA c ; 5‐hydroxytryptamine‐3A; NR1a/NR2A NMDA; GluR1/GluR2 AMPA; GluR6/KA2 kainate; and α 4 β 2 , α 4 β 4 , and α 2 β 4 nicotinic‐acetylcholine] and the G‐protein–coupled inward rectifying potassium channel GIRK2. We also investigated the effect of acetaldehyde on the dopamine transporter (DAT), performing dopamine uptake assays in oocytes expressing DAT. Results: Acetaldehyde (1 and 10 μM) significantly enhanced α 1 glycine receptor–mediated currents. Acetaldehyde did not affect the function of any of the other receptors tested or the potassium currents measured in GIRK2 channels. Moreover, acetaldehyde did not alter the DAT‐mediated dopamine uptake. Conclusions: Our results suggest a potential minor role for acetaldehyde in the glycine receptor–mediated effects of ethanol. Otherwise, acetaldehyde does not modulate function of the neuronal receptors tested in this study, in GIRK channels or DAT, when expressed recombinantly in Xenopus laevis oocytes.