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Association Study Between Genetic Polymorphisms in the 14–3‐3 η Chain and Dopamine D4 Receptor Genes and Alcoholism
Author(s) -
Ishiguro H.,
Saito T.,
Shibuya H.,
Arinami T.
Publication year - 2000
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.2000.tb04619.x
Subject(s) - dopaminergic , genetics , exon , gene , dopamine receptor , genetic association , single nucleotide polymorphism , dopamine , polymorphism (computer science) , mutation , biology , receptor , genotype , endocrinology
Background: The dopaminergic system may be involved in the development of alcoholism. As part of our ongoing studies on the association between alcoholism and dopaminergic genes, we report herein a mutation analysis of the 14–3‐3 η chain gene ( YWHAH ) and an association study between alcoholism and the YWHAH and dopamine D4 receptor gene ( DRD4 ) polymorphisms. Methods: Nucleotide mutations were investigated using single‐strand conformation polymorphism methods. Associations were analyzed using a case‐control design involving 185 Japanese alcoholics and 286 Japanese controls. Results: Five polymorphisms, ‐147G>A, ‐134(GCCTGCA)2–4, IVS1+31(G)7–8. IVS1+73–74ins(G), and 753A>G, were detected on the YWHAH , and three of them were novel. No significant associations were found between alcoholism and these polymorphisms or two additional polymorphisms on DRD4 exon III and DRD4 ‐521C/T. Conclusions:YWHAH and DRD4 do not appear to play a major role in the development of alcoholism.