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Evidence for Ethanol Oxidation by Kupffer Cells
Author(s) -
Nakamura Yuji,
Yokoyama Hirokazu,
Okamura Yukishige,
Ohgo Hideki,
Fukuda Masahiko,
Horie Yoshinori,
Kato Shinzo,
Ishii Hiromasa
Publication year - 1999
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1999.tb04542.x
Subject(s) - acetaldehyde , aldehyde dehydrogenase , ethanol , kupffer cell , chemistry , disulfiram , biochemistry , alcohol dehydrogenase , ethanol metabolism , enzyme , medicine
Ethanol oxidation in Kupffer cells was investigated by measuring 14 C‐acetate formation from 14 C‐ethanol, and the role of aldehyde dehydrogenase 2 (ALDH2) in this process was also examined. Formation of 14 C‐acetate from 20 mM of 14 C‐ethanol was significantly increased in medium‐containing Kupffer cells (9,003 + 2,066 cpm/5 × 10 6 cells), compared with medium containing no cells (1,826 + 46 cpm, p < 0.01), or containing acid‐killed Kupffer cells (1,629 + 210 cpm, p < 0.01). Ethanol formation was significantly attenuated when 20 and 200 μM cyanamide or 2 μM disulfiram were given. Reverse transcriptase‐polymerase chain reaction demonstrated that Kupffer cells carry mRNA for ALDH2. These findings indicate that Kupffer cells can oxidize ethanol to acetate. ALDH2 may participate in this process, especially in the conversion of acetaldehyde to acetate.