Premium
Relationship Between Serum Levels of Anti‐Low‐Density Lipoprotein‐Acetaldehyde‐Adduct Antibody and Aldehyde Dehydrogenase 2 Heterozygotes in Patients With Alcoholic Liver Injury
Author(s) -
Nagata Naruhiko,
Watanabe Norihito,
Tsuda Michio,
Tsukamoto Hideo,
Matsuzaki Shohei
Publication year - 1999
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1999.tb04529.x
Subject(s) - acetaldehyde , aldehyde dehydrogenase , antibody , adduct , heterozygote advantage , chemistry , lipoprotein , liver injury , aldehyde , endocrinology , biochemistry , medicine , enzyme , immunology , ethanol , gene , cholesterol , organic chemistry , genotype , catalysis
We prepared low‐density lipoprotein (LDL)‐acetaldehyde‐adduct (hereafter abbreviated as LDL‐adduct) and anti‐LDL‐adduct antibody by using Watanabe hyperlipidemic rabbits, and determined values of serum anti‐LDL‐adduct antibody levels by the ELISA method in healthy adults and patients with alcoholic liver injury. In the nondrinking group in healthy adults, values of anti‐LDL‐adduct antibody levels were 25 ± 13 μ g/ml, and there was no significant difference between moderate drinkers without diseases and the nondrinking group in healthy adults. Values of anti‐LDL‐adduct antibody in alcoholic disease groups, 17 ± 9 μ g/ml for the patients with the fatty liver group, 21 ± 14 μ g/ml for the hepatic fibrosis group, 70 ± 21 μ g/ml for the alcoholic hepatitis group, 41 ± 50 μ g/ml for the alcoholic cirrhosis group, and 19 ± 18 μ g/ml for the alcoholic pancreatitis group. Examinations of aldehyde dehydrogenase 2 (ALDH2) genetic variations by the polymerase chain reaction‐single‐strand conformation polymorphism (PCR‐SSCP) method in the healthy group and the liver injury group revealed a tendency for patients with ALDH2 1 /2 2 in the liver injury group to have relatively mild liver lesions. When comparing anti‐LDL‐adduct antibody levels between ALDH2 genetic variations, those for the patients with ALDH2 1 /2 1 (36 ± 40 μ g/ml) were significantly higher than those for patients with ALDH 1 /2 2 (11 ± 5 μ g/ml). Results of the present study suggest that genetic variation may influence the progression of liver injury.