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Chronic and Acute Prenatal and Postnatal Ethanol Exposure on Lymphocyte Subsets from Offspring Thymic, Splenic, and Intestinal Intraepithelial Sources
Author(s) -
Basham Karen B.,
Whitmore Sarah P.,
Adcock Audrey F.,
Basta Patricia V.
Publication year - 1998
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1998.tb03942.x
Subject(s) - offspring , intraepithelial lymphocyte , spleen , lymphocyte , immunology , fetus , flow cytometry , pregnancy , immune system , physiology , medicine , biology , andrology , endocrinology , genetics
The overall objective of this study was to analyze the effects of a combined prenatal and postnatal (entire gestational human chronic drinking model) ethanol exposure on T‐cell development in mice. Specifically, this study evaluated the effects of chronic exposure to prenatal ethanol on lymphocyte makeup and proliferative capabilities of postnatal offspring's (4 and 12 weeks) peripheral lymphoid tissues. Chronic exposure regimens were conducted over the entire gestational period and through postnatal day 14 or 21. Thymus, spleen, and intestinal intraepithelial lymphocytes were harvested and analyzed by flow cytometry for percentages of T‐cell subsets. Splenic lymphocytes were also analyzed for their ability to proliferate in response to a T‐cell mitogen. Limited effects of chronic ethanol exposure were seen.