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Varying Effects of Ethanol on Transfected Cell Lines
Author(s) -
Asai Kiyofumi,
Ishii Akira,
Yao Una,
Diamond Ivan,
Gordon Adrienne S.
Publication year - 1998
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1998.tb03633.x
Subject(s) - transfection , cell culture , ethanol , microbiology and biotechnology , cell , chemistry , plasmid , biology , biochemistry , gene , genetics
The use of transfected cell lines has provided a powerful approach to study the functions of transfected proteins. This approach has also proven useful to determine the effects of acute and chronic ethanol exposure on particular proteins. We show here, however, that the effects of ethanol on transfected proteins vary between transiently transfected cells and stably transfected cell lines. Moreover, we found that the effect of ethanol on a particular protein depends on the plasmid vectors used for the transfection.