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Effects of Chronic Ethanol on the Mobilization of Arachidonate and Docosahexaenoate Stimulated by the Type 2A Serotonin Receptor Agonist (±)‐2,5‐Dimethoxy‐4‐iodoamphetamine Hydrochloride in C6 Glioma Cells
Author(s) -
Garcia Martha C.,
Kim KunYang,
Hough Christopher,
Kim HeeYong
Publication year - 1997
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1997.tb04477.x
Subject(s) - arachidonic acid , chemistry , agonist , docosahexaenoic acid , serotonin , polyunsaturated fatty acid , calcium , pharmacology , hydrochloride , 5 ht receptor , calcium in biology , ethanol , receptor , medicine , endocrinology , biochemistry , fatty acid , biology , organic chemistry , enzyme
We studied the effects of chronic ethanol exposure on the mobilization of polyunsaturated fatty acids stimulated by activation of the type 2A serotonin receptor in C6 glioma cells. In our in vitro model, we prelabeled cells with [ 3 H]arachidonate and [ 14 C]docosahexaenoate and subsequently stimulated with the type 2A serotonin receptor agonist (±)‐2,5‐dimethoxy‐4‐iodoamphetamine hydrochloride. In as early as 10 days of exposure to 20 or 50 mM ethanol, the (±)‐2,5‐dimethoxy‐4‐iodoamphetamine hydrochloride‐stimulated mobilization of [ 3 H]arachidonic acid ([ 3 H]AA) and [ 14 C]docosahexaenoic acid ([ 14 C]DHA) was significantly inhibited, and this inhibition was accompanied by decreased mobilization of intracellular [Ca 2+ ] i . Exposure to ethanol did not alter significantly the release of [ 3 H]AA and [ 14 C]DHA stimulated by the calcium ionophore A23187 nor the incorporation of [ 3 H]AA and [ 14 C]DHA into cellular lipids. Decreased mobilization of polyunsaturated fatty acids and calcium in astroglia may contribute to neurotoxicity caused by chronic ethanol exposure.