Premium
Activation of Arachidonic Acid‐Specific Phospholipase A 2 in Human Neuroblastoma Cells after Chronic Alcohol Exposure: Prevention by GM1 Ganglioside
Author(s) -
Basavarajappa Balapal S.,
Saito Mitsuo,
Cooper Thomas B.,
Hungund Basalingappa L
Publication year - 1997
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1997.tb04438.x
Subject(s) - arachidonic acid , ganglioside , alcohol , phospholipase , chemistry , phospholipase a2 , biochemistry , medicine , endocrinology , enzyme
Human neuroblastoma cells were exposed to ethanol (EtOH; 100 mM) in culture for various time periods. It was found that chronic EtOH exposure increased the arachidonyl‐specific phospholipase A 2 (PLA 2 ) activity significantly in both cytosol (1.6‐fold) and membrane (2.2‐fold) fractions when 1‐palmitoyl‐2‐arachidonyl‐ sn ‐glycero‐3‐phosphocholine was used as a substrate. This arachidonyl‐specific PLA 2 activity progressively increased with increasing duration of EtOH exposure and reached peak level at 72‐hr EtOH exposure (chronic). A significant amount of the PLA 2 activity was associated with the membrane fraction. No significant difference in PLA 2 activity was observed when 1‐palmitoyl‐2 oleoyl or linoleoyl‐ sn ‐glycero‐3‐phosphocholine was used as a substrate. It was also found that co‐treatment of neuroblastoma cells with ganglioside GM1 reduced the EtOH‐induced activation of arachidonyl‐specific PLA 2 activity. The present results indicate that arachidonic acid‐specific PLA 2 may play a role in adaptation mechanism to chronic EtOH in cultured neuroblastoma cells. Ganglioside GM1, in part, may exert its neuroprotective effects by modulating arachidonyl‐specific PLA 2 activity in chronic EtOH‐exposed neuroblastoma cells.