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Genetic Polymorphism of Cytochrome P‐4502E1 and Mitochondrial Aldehyde Dehydrogenase in a Korean Population
Author(s) -
Lee KiHwan,
Kwak BoYeon,
Kim JongHo,
Yoo SeungKu,
Yum SungKwan,
Jeong HanSeung
Publication year - 1997
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1997.tb04236.x
Subject(s) - aldh2 , aldehyde dehydrogenase , allele , microbiology and biotechnology , genotype , biology , genetics , restriction fragment length polymorphism , acetaldehyde , polymerase chain reaction , genotyping , allele frequency , mitochondrial dna , gene , biochemistry , ethanol
Mitochondrial aldehyde dehydrogenase (ALDH2) is mainly responsible for the oxidation of acetaldehyde generated during alcohol oxidation in vivo. Cytochrome P‐4502E1 (CYP2E1), a liver microsomal enzyme, also metabolizes acetaldehyde and ethanol. Genetic polymorphism of ALDH2 and CYP2E1 was investigated among 481 Korean adults. A new restriction fragment‐length polymorphism method was developed to determine the genotype of the ALDH2 alleles. This method proved to be simpler and faster than the hybridization method using allele‐specific oligonucleotide probes and polymerase chain reaction‐directed mutagenesis. The allele frequencies of ALDH2 1 and ALDH2 2 were 0.840 and 0.160, respectively. This allele frequency of ALDH2 2 is less than in Japanese people. Genetic polymorphism of CYP2E1 was investigated using polymerase chain reaction and restriction fragment‐length polymorphism. The estimated allele frequencies for C1 and c2 were 0.808 and 0.192.