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Acute Ethanol Effects on Focal Cerebral Ischemia in Nonfasted Rats
Author(s) -
Zhao YongJie,
Yang GuoYuan,
Domino Edward F.
Publication year - 1997
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1997.tb03832.x
Subject(s) - ischemia , ethanol , cerebral edema , middle cerebral artery , medicine , in vivo , anesthesia , occlusion , endocrinology , chemistry , biochemistry , biology , microbiology and biotechnology
Focal cerebral ischemia was induced in a rat model of middle cerebral artery occlusion. Three groups of adult male Sprague‐Dawley rats, given food and water ad libitum, were subjected to 4 hr of middle cerebral artery occlusion. All were given vehicle control and ethanol pretreatments intraperitoneally 1 hr before. Mean ipsilateral brain water content in the control, 2 g/kg ethanol, and 2 g/kg ethanol + insulin‐treated groups showed ischemia core: 81.1%, 82.5%, and 80.9%; intermediate zone: 81.0%. 81.9%, and 80.3%; and outer zone: 80.3%, 81.3%. and 80.1%, respectively. Brain Na + and K + content in these groups paralleled the water content. In addition to significantly ( p < 0.05) more brain edema, the 2 g/kg ethanol‐treated animal group also had significant hyperglycemia. In contrast, the 2 g/kg ethanol + insulin‐treated animals were normoglycemic and had ischemic, intermediate, and outer zone Na + , K + , and CI − levels comparable with the control group ( p > 0.05). These results stress the importance of measuring and controlling plasma glucose levels in the in vivo studies of the neurotoxic effects of acute ethanol.