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Chronic Ethanol Downregulates Gal‐β‐l,4GlcNAc α2,6‐Sialyltransferase and Gal‐β‐l,3GlcNAc α2,3‐Sialyltransferase mRNAs in Rat Liver
Author(s) -
Rao Manjunath N.,
Lakshman M. R.
Publication year - 1997
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1997.tb03771.x
Subject(s) - medicine , ethanol , endocrinology , sialyltransferase , chemistry , enzyme , biology , biochemistry
We have previously demonstrated that chronic ethanol specifically decreases the hepatic level and rate of synthesis of 2,6‐sialyltrans‐ferase (2,6‐ST). To understand its mechanism of action, effects of 8 weeks of chronic ethanol feeding on the expression of sialyltrans‐ferase (ST) genes in rat liver and kidneys were determined by North‐emblot analysis of ST mRNAs. It was found that, compared with the pair‐fed control rats, the percentage decreases in ST mRNAs in the ethanol‐fed group were as follows: liver—Gal‐β‐1,4GlcNAc α2,6‐ST (2,6‐ST): 59% (p < 0.001); liver—Gal‐β‐1,3GlcNAc α2,3‐ST (2,3‐ST): 32% (p < 0.01); and kidneys—2,6–ST: 5% (NS). In contrast, glyceral‐dehyde‐3‐phosphate dehydrogenase mRNA in both liver and kidneys was unaffected by the same ethanol treatment. Taken together, these results demonstrate that chronic ethanol downregulates the expression of 2,6‐ST and 2,3‐ST genes in rat liver.