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Protective Mechanism of High‐Density Lipoprotein against Endotoxemia in Chronic Alcohol Ingestion
Author(s) -
Kitano Hiroyuki,
Fukui Hiroshi,
Okamoto Yasuyuki,
Kikuchi Eiryou,
Matsumoto Masami,
Kikukawa Masaji,
Morimura Masafumi,
Tsujita Sigenobu,
Nagamoto Issei,
Hoppo Kazushige,
Nakatani Yoshihiro,
Nakatani Toshiya,
Tsuji Tadasu
Publication year - 1996
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1996.tb01807.x
Subject(s) - medicine , ethanol , lactate dehydrogenase , endocrinology , ingestion , tumor necrosis factor alpha , alcohol dehydrogenase , lipoprotein , chemistry , high density lipoprotein , cholesterol , biochemistry , enzyme
In the present study, we evaluated the role of high‐density lipoprotein (HDL) as an endotoxin‐binding protein in chronically alcohol‐fed rats. Although the blood endotoxin level was significantly elevated in chronic ethanol‐loaded rats, compared with control rats, serum tumor necrosis factor (TNF), ALT, and lactate dehydrogenase were not elevated. Serum HDL and its endotoxin‐binding capacity were significantly increased in chronic ethanol‐loaded rats. When Kupffer cells isolated from control and chronic ethanol‐loaded rats were cultured in the medium containing 3 to 30 mg/dl HDL and endotoxin (500 ng/ml), endotoxin uptake and TNF production of Kupffer cells were decreased in proportion to the concentration of HDL in the medium. These results suggest that the increase in endetoxin‐binding capacity of HDL may serve as a protective mechanism against endotoxin in chronic ethanol‐loaded rats.