Premium
Identification of Cholesta‐3,5‐dien‐7‐one by Gas Chromatography‐Mass Spectrometry in the Erythrocyte Membrane of Alcoholic Patients
Author(s) -
Adachi Junko,
Hojo Keiko,
Ueno Yasuhiro,
Naito Takeaki,
Ninomiya Ichiya,
Imamichi Hiroyuki,
Tatsuno Yoshitsugu
Publication year - 1996
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1996.tb01729.x
Subject(s) - docosahexaenoic acid , erythrocyte membrane , arachidonic acid , chromatography , mass spectrometry , gas chromatography , chemistry , polyunsaturated fatty acid , gas chromatography–mass spectrometry , membrane , biochemistry , fatty acid , enzyme
Lipids and oxidized lipids were analyzed by gas chromatography‐mass spectrometry in the erythrocyte membranes of alcoholic and control subjects. Cholesta‐3,5‐dien‐7‐one and cholesta‐trienes were detected in alcoholic samples examined, but not in significant amounts in controls. Levels of polyunsaturated fatty acids (arachidonic acid, 20:4; docosahexaenoic acid, 22:6; and docosatetraenoic acid, 22:4) in alcoholic samples declined significantly, whereas cholesta‐3,5‐dien‐7‐one levels increased. A high level of total bilirubin was observed in most patients. A possible mechanism of the accumulation of choletsa‐3,5‐dien‐7‐one in the erythrocyte membrane of alcoholics is discussed.