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Ethanol Consumption Reduces the Cytolytic Activity of Lymphokine‐Activated Killer Cells
Author(s) -
Gallucci Randle M.,
Meadows Gary G.
Publication year - 1995
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1995.tb01522.x
Subject(s) - cytolysis , lymphokine , lymphokine activated killer cell , immunology , chemistry , microbiology and biotechnology , biology , immune system , cytotoxicity , t cell , in vitro , biochemistry , interleukin 21
Ethanol (20% w/v) given to female C57BL/6 mice in their drinking water reduces splenic natural killer (NK) cell cytolytic activity after 2, 4, and 10 weeks of consumption. This reduction is transient because the levels of NK cell cytotoxicity from ethanol‐consuming mice are nearly equal to those of water‐drinking mice after splenocytes were incubated in 1000 lU/ml of recombinant interleukin‐2 (rlL2) for 16–18 hr. In this study, mice were given 20% w/v ethanol in the drinking water for 2 weeks. Splenic NK cells were enriched up to 88% by negative selection based on surface expression of NK1.1. Enriched NK cells were expanded in HL2 for 6 days. Lymphokine‐activated killer (LAK) cells from both ethanol‐consuming and water‐drinking mice were <95% NK1.1 + . LAK cell cytolytic activity was significantly lower against NK‐insensitive P815 mastocytoma [6.67 ± 2.18 vs. 17.21 ± 1.8 lytic units (LUs), p < 0.01], moderately NK‐sensitive B16 melanoma (25.3 ± 6.6 vs. 66.2 ± 14.2 LU, p < 0.05), and NK‐sensitive YAC‐1 lymphoma targets (80.5 ± 34.7 vs. 177.0 ± 43.6 LU, p < 0.005) in cells from ethanol‐consuming mice compared with water‐drinking controls. Ethanol consumption did not affect the morphology or phenotype of LAK cells with respect to surface expression of NK1.1, B220, CD3, CD25, CD11a, CD54, CD45RB, or class I major histocompatibility complex.