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Use of Repeated Measures in an Analysis of Ethanol‐Induced Loss of Righting Reflex in Inbred Long‐Sleep and Short‐Sleep Mice
Author(s) -
Markel Paul D.,
DeFries John C.,
Johnson Thomas E.
Publication year - 1995
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1995.tb01506.x
Subject(s) - righting reflex , analysis of variance , inbred strain , sleep (system call) , sensitization , medicine , repeated measures design , reflex , anesthesia , audiology , psychology , biology , immunology , genetics , statistics , mathematics , computer science , gene , operating system
We present a repeated‐measures analysis of ethanol‐induced loss of righting reflex (LRR) in Inbred Long‐Sleep (ILS) and Inbred Short‐Sleep (ISS) strains of mice and their F 1 and F 2 cross progeny. Mice were administered a 4.1 g/kg intraperitoneal dose of ethanol at two times, 7–10 days apart. Repeatability is nonsignificant in ILS, ISS, and F, mice, but is highly significant (0.47, p < 0.01) in the F 2 mice. Mean LRR does not differ between trials 1 and 2, with the exception of the ISS strain in which the interaction of sex with LRR sensitization is significant. This two‐trial method leads to increased accuracy of genotype assessment for pharmacological or behavioral traits where trial 1 does not influence the outcome of trial 2. The repeated‐measures design facilitates novel analyses of the duration of LRR, and results suggest that most environmental variance for LRR is due to nonreplicable influences.