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Alcohol Inhibits Epidermal Growth Factor‐Stimulated Progesterone Secretion from Human Granulosa Cells
Author(s) -
McKenzie Pamela P.,
McClaran Joseph D.,
Caudle Michael R.,
Fukuda Aisaku,
Wimalasena Jay
Publication year - 1995
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1995.tb00996.x
Subject(s) - medicine , endocrinology , epidermal growth factor , luteinizing hormone , gonadotropin , secretion , stimulation , menotropins , biology , gonadotropin releasing hormone , hmg coa reductase , hormone , chemistry , receptor , ovulation , ovulation induction , biochemistry , enzyme , reductase
In this study, luteinized human granulosa cells (GC) obtained during in vitro fertilization procedures were used as a model system to evaluate the effects of ethanol (EtOH), a well‐known reproductive toxin, on epidermal growth factor (EGF) and gonadotropin‐stimulated steroidogenesis. Our results demonstrate that the basal progesterone (P 4 ) and estradiol (E 2 ) secretion by human GC in vitro was dependent on the ovarian stimulation protocol. EGF significantly enhanced P 4 , but not E 2 , secretion in human GC from clomiphene citrate (CC), human menopausal gonadotropin (hMG), and hMG/gonadotropin‐releasing hormone agonist (GnRH‐a)‐treated patients. The effects of EGF plus luteinizing hormone (LH) were additive in cells from the CC group, but less than additive in hMG and hMG/GnRH‐a groups. EtOH at 20 mM or more inhibited EGF stimulated P 4 secretion in human GC from all three patient groups. EtOH inhibited P 4 secretion stimulated by EGF and LH cotreatment in the CC and hMG/GnRH‐a groups, but not in human GC from the hMG‐treated patients. These results suggest that basal and EGF or LH‐stimulated P 4 secretion by human GC, as well as the effects of EtOH, are profoundly influenced by the follicle's hormonal milieu.