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Macrophages Are a Major Source of Acetaldehyde in Circulating Acetaldehyde‐Albumin Complexes Formed after Exposure of Mice to Ethanol
Author(s) -
Wickramasinghe S. N.,
Mawas F.,
Hasan R.,
Brown I. N.,
Goldin R. D.
Publication year - 1994
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1111/j.1530-0277.1994.tb01451.x
Subject(s) - acetaldehyde , ethanol , chemistry , cytotoxicity , albumin , serum albumin , cytotoxic t cell , biochemistry , in vitro
C57BL mice were depleted of macrophages by an intravenous injection of liposome‐encapsulated dichloromethylene diphosphonate (DCMDP), and control mice were uninjected or injected with empty liposomes. One day after injection, a proportion of the DCMDP‐treated and control mice was continuously exposed to ethanol vapor for 4 days. Albumin fractions were separated from the sera of both ethanol‐unexposed and ethanol‐exposed animals and tested for cytotoxicity against a monolayer of A9 cells using two indicators of cytotoxicity: detachment of adherent cells and a decrease in the ability of cells to reduce tetrazolium. The results show that, in mice exposed to ethanol, macrophages are a major source of the acetaldehyde in circulating cytotoxic acetaldehyde‐albumin complexes and presumably also of free acetaldehyde.